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Cellular uptake of MSC-derived EVs imaged in live NSC culture. (a–b) Confocal microscopy imaging of live DiO-labelled NSCs (green) with nuclear counterstain (blue), incubated in the (a) presence or (b) absence of DiD-labelled EVs. Arrows show DiD-labelled EVs in magnified view of boxed area. (c) SIM imaging of DiD-labelled EVs (red) uptake by DiO-labelled NSCs (green), with magnified areas 1 and 2 showing intracellular DiD-labelled EVs (arrows).
