Fig. 2.

Sequence similarity and structural homology of Pfs48/45 6C. a Sequence alignment of Pfs48/45 6C with proteins of highest sequence identity (Pvs48/45, Pms48/45, Pos48/45, and Pf47) and structural homology (Pf12, Pf41, and Sporosag). Secondary structure elements of Pfs48/45 6C are shown atop the sequence alignment, with an arrow and a cylinder indicative of a strand and a helix, respectively. Orange dots above the Pfs48/45 sequence indicate residues contacted by mAb 85RF45.1. b Schematic diagram of Pfs48/45 showing a five-on-four ß sandwich disposition with parallel and anti-parallel ß-strands. Disulfide bonds are indicated by red lines. c Secondary structure cartoon representation of Pfs48/45 6C in comparison to known structures of highest homology (PDB IDs: 2YMO [https://www.rcsb.org/structure/2YMO]⁵⁵, 4YS4 [https://www.rcsb.org/structure/4YS4]⁵⁶, 2WNK [https://www.rcsb.org/structure/2WNK]⁵⁷). Disulfide bonds are represented as red sticks. Root mean square deviations (rmsd) between Pfs48/45 and the structures as calculated by the DALI server³¹ are indicated in parentheses