Table 1.
Sequences of primers and TaqMan probes used in polymerase chain reaction (PCR)
| Sequences |
| Multiplex real‐time PCR I |
| Aggregatibacter actinomycetemcomitans |
| Primer F GCGAAACGAAGAGAAGCAAG |
| Primer R CCTACCCAACAGGCGTATCA |
| Probe FAM‐ATTCCCAACCGCACTT‐BHQ1 |
| Treponema denticola |
| Primer F GTTGTTCGGAATTATTGG |
| Primer R GATTCAAGTCAAGCAGTA |
| Probe Texas Red‐TCACACCAGGCTTACC‐BHQ |
| Fusobacterium nucleatum |
| Primer F GGCTTCCCCATCGGCATTCC |
| Primer R AATGCAGGGCTCAACTCTGT |
| Probe CY5‐TCCGCTTACCTCTCCAG‐BHQ3 |
| Multiplex real‐time PCR II |
| Porphyromonas gingivalis |
| Primer F CTGCGTATCCGACATATC |
| Primer R GGTACTGGTTCACTATCG |
| Probe FAM‐ACCATAGACGACGGAGCACC‐BHQ1 |
| Tannerella forsythia |
| Primer F GAGGTTGTGGAAGGTATG |
| Primer R GTAGATCAGAATGTACGGATT |
| Probe Texas Red‐TCTCCGCTTATTTCGTGAC‐BHQ2 |
| Streptococcus salivarius |
| Primer F CACGCCATGCTGGAAGTG |
| Primer R GCGATGAGCCAAGCTGAAG |
| Probe CY5‐TTAGCTGCTGCGTAGACTTCGTCT‐BHQ3 |
| Solobacterium moorei |
| Primer F CGGGTGAGTAATACATAAGCAAC |
| Primer R ACCTTTAATAACCAGAAGATGCCT |
| Probe HEX‐TGGGATAATCTCTGGAAACGGGGACT‐BHQ1 |