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. 2018 Oct 26;8:15869. doi: 10.1038/s41598-018-34119-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Culture conditions on CPP-PEDOT substrate with soluble fibronectin factor for hADSC sheet formation. Photothermal images of PP-PEDOT (a) and SP-PEDOT (b) in the same conditions (I_pw = 2.7 W/cm², t_nir = 3 min, RD = 41.4J). (c) UV/Vis/NIR spectrum of PP-PEDOT (red) and SP-PEDOT (black) substrate (red arrow means 808 nm). After the cells were cultured for 1 day (cell seeding number = 100,000 cells/dish), optical images of the adherent hADSCs on the CPP-PEDOT substrate (d) without soluble fibronectin and (e) with soluble fibronectin (red arrows mean areas of concentrated cells). (f) Effect of harvesting efficiency (ε_d) and sheet area (A_hcs) at different fibronectin concentrations (1 μg/ml to 1 pg/ml, P_sf means the point of sheet formation). (g) Schematic image of the function of soluble fibronectin in hADSC culture conditions. (h–j) Culture conditions on CSP-PEDOT substrate for hADSC sheet formation. (h) Optical image of the adherent hADSCs on the CPP-PEDOT substrate at 1 day. (i) Optical image of the detached sheet fragments from the CPP-PEDOT substrate at 1 day. (j) Effect of the cell adhesion rate and the thickness of collagen layer.

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