Figure 2.
E-cadherin expression of the adherent hADSCs on the CPP-PEDOT substrate and intercellular distance of the harvested cell sheets at different cell concentrations. With 10−8% (100 pg/ml) soluble fibronectin molecules, DAPI/E-cadherin images of the adherent hADSCs on the substrate at different cell concentrations; cell seeding numbers were (a,d) 1.8 × 105 cells/dish, (b,e) 2.7 × 105 cells/dish, and (c,f) 3.7 × 105 cells/dish. DAPI-stained images of the harvested sheets with different cell seeding numbers: 400,000 cells/dish (g), 800,000 cells/dish (h), and 1,200,000 cells/dish (i). Graphs of the calculated intercellular distance from the DAPI data at 400,000 cells/dish (j), 800,000 cells/dish (k), and 1,200,000 cells/dish (l). The cell groups are randomly selected cell groups in the same area of the cell sheet. Each cell groups in x-axis are consisting of two cells to measure intercellular distance between the two cells(y-axis).