Table 1.
Culture conditions of the hADSC sheet on the CPP-PEDOT substrate with different concentration of soluble fibronectin factor and cells.
FN concentrationa | Cell concentration | Adet (≤Anir)b [mm2] | εeff.dc [%] | Ahcsd [mm2] | IDdete [μm] |
---|---|---|---|---|---|
Control | 4 × 105 | 132.7 | 100 | 1.89 | — |
10−4% (1 μg/ml) | 4 × 105 | 0 | 0 | 0 | — |
10−5% (100 ng/ml) | 4 × 105 | 0 | 0 | 0 | — |
10−6% (10 ng/ml) | 4 × 105 | 132.7 | 100 | 7.74 | — |
10−7% (1 ng/ml) | 4 × 105 | 132.7 | 100 | 15.1 | — |
10−8% (100 pg/ml) | 4 × 105 | 132.7 | 100 | 26.8 | 13.7 |
10−8% (100 pg/ml) | 8 × 105 | 132.7 | 100 | 63 | 13.1 |
10−8% (100 pg/ml) | 1.2 × 106 | 132.7 | 100 | 72.8 | 12.6 |
10−8% (100 pg/ml) | 1.4 × 106 | 471.5 | 100 | 122.6 | 13.5 |
10−9% (10 pg/ml) | 4 × 105 | 132.7 | 100 | 10.2 | — |
aConcentration of fibronectin protein, bdetached area of PEDOT substrate, charvesting efficiency of detached area, ddetached area of hADSC sheet, eintercellular distance of detached hADSC sheet. All sample were detached at the same input power density (2.7 W cm−2) and irradiated area of NIR laser (132.7 mm2).