Skip to main content
. 2018 Oct 26;9(11):1097. doi: 10.1038/s41419-018-1061-4

Fig. 6. KD prevents increase in IL-1α and superoxide, but not IL-1β, and preserves axons and VEP.

Fig. 6

a Average mouse weight over duration of the study. Mice on the KD lost weight over the course of the first week, but regained this weight over time. Both diet groups had comparable weight at the beginning and end of the study. b Quantification of ketone body levels in serum of mice fed a KD or KCD. The KD increased ketone plasma levels regardless of blast exposure. c Representative western blot of SOD2. d Quantification of SOD2 levels in retinas from sham or post-blast mice fed control (KCD) or KD (Keto) diet. The KD prevented the blast-induced decrease in SOD2. e Quantification of DHE fluorescence in the retinas of mice on the KD or KCD. The KD prevented the increase in superoxide levels. f Quantification of cleaved to total caspase-1 in retinas from sham or post-blast mice fed a KCD or KD. The KD prevented activation of caspase-1. g Quantification of IL-1α levels in retinas from sham or post-blast mice fed a KD or KCD. The KD prevented the blast-induced increase in IL-1α. h Quantification of IL-1β levels in retinas from sham or post-blast mice fed a KD or KCD. Levels were increased similarly in both groups. i-k Brightfield micrographs of representative optic nerve cross-sections from KCD sham (i), KCD 4 weeks post-blast (j), and KD 4 weeks post-blast (k) mice. l Quantification of ON cross-sectional glial area in all groups showing an increase in glial area only in the KCD group. m Quantification of total axons in all groups showing a decrease only in the KCD group. n Quantification of CTB fluorescence in the superior colliculus (percent axon transport) in mice from all groups showing a decreased loss in transport in the KD group. o, p Quantification of VEP N1 amplitude (o) and latency (p) in sham and post-blast mice on all diets showing preservation of waveforms in the KD group. A total of 5 samples were used for the biochemistry and axon histology. A total of 8 KCD sham mice, 10 KCD blast mice, 5 KD sham mice, and 11 KD blast mice were used for the VEPs. *p < 0.05, **p < 0.01, ***p < 0.001