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. 2018 Apr 24;99(4):749–760. doi: 10.1093/biolre/ioy099

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RNA interference of FDPS and GGPS1. (A–F) Endometrial stromal cells were transfected with scramble siRNA or siRNA targeting FDPS or GGPS1 for 48 h. Cells were incubated for 24 h in serum-free medium and then challenged with control medium black bars () or 100 HU/ml pyolysin red bars () for 2 h. The mRNA expression of each cognate gene was measured by qPCR and normalized to two reference genes, ACTB and RPL19 (A, D). Data are presented as mean (SEM); n = 3 animals. Data were analyzed by Student t-test; values differ from scramble, * P < 0.05, *** P < 0.001. Cell viability was quantified by MTT assay and data expressed as percent of control (B, E), and supernatants were collected to measure the leakage of LDH from cells and data expressed as percent of control challenged with pyolysin (C, F). Data are presented as mean (SEM); n = 5 animals. Data were analyzed by one-way ANOVA and Dunnett multiple comparison post hoc test; values differ from scramble challenged with pyolysin, *** P < 0.001, NS = not significant. (Please see the online version for the color figure.)

Inline graphic — RNA interference of FDPS and GGPS1. (A–F) Endometrial stromal cells were transfected with scramble siRNA or siRNA targeting FDPS or GGPS1 for 48 h. Cells were incubated for 24 h in serum-free medium and then challenged with control medium black bars () or 100 HU/ml pyolysin red bars () for 2 h. The mRNA expression of each cognate gene was measured by qPCR and normalized to two reference genes, ACTB and RPL19 (A, D). Data are presented as mean (SEM); n = 3 animals. Data were analyzed by Student t-test; values differ from scramble, * P < 0.05, *** P < 0.001. Cell viability was quantified by MTT assay and data expressed as percent of control (B, E), and supernatants were collected to measure the leakage of LDH from cells and data expressed as percent of control challenged with pyolysin (C, F). Data are presented as mean (SEM); n = 5 animals. Data were analyzed by one-way ANOVA and Dunnett multiple comparison post hoc test; values differ from scramble challenged with pyolysin, *** P < 0.001, NS = not significant. (Please see the online version for the color figure.)