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. 2018 May 3;99(4):789–797. doi: 10.1093/biolre/ioy105

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Figure 4. — Effects of Enkur genotype on flagellar bending. Flagellar motility of uncapacitated cauda epididymal sperm from Enkur^+/+ (+/+, uncap) and Enkur^tm/tm (tm/tm, uncap) mice were examined and compared with Enkur^+/+ following incubation under capacitating conditions in vitro (+/+, cap). (A) A representative montage of flagellar bends (+/+uncap, a1-j1; tm/tm uncap, a2-j2; +/+ cap, a3-j3) with about 33 ms elapsed between frames. (B) Traces obtained from montage shown in Panel A. Large circles mark the point where the flagellum contacts the base of the head. (C) Primary bend amplitudes of cauda epididymal sperm from Enkur^+/+ (+/+) and Enkur^tm/tm (tm/tm) mice at the beginning of incubation under capacitating conditions and after 90-min incubation. Data represent means ± SD (horizontal line above bars represents statistical differences by two tailed t-test).