Table 2.
Summary of IHC-relevant guidelines in BOR.
Data Type | Specifications/guidelines |
---|---|
Immunohistochemistry/Immunofluorescence | Appropriate, representative controls need to be reported (either in the figures or in supplemental data) |
Antibodies | • Supplementary antibody table |
• Materials and methods | |
- Positive and negative controls | |
- Validation information | |
- Lot number | |
- Associated references | |
- RRID | |
• Antibody registry [10] | |
- If antibody is not registered, then author needs to register and obtain an RRID by the revision stage of manuscript submission | |
BOR endorsed reference | Saper C.B. An open letter to our readers on the use of antibodies. J Comp Neurol 2005. 493(4):477–488. |