Persistence length for systems with uniform linker length = 18 bp, uniform linker length = 27 bp, life-like, life-like with NFRs, and gene encoding-like fibers. Persistence length is calculated according to Eq. 2. A short persistence length is associated with a more flexible fiber. NFRs decrease the persistence length by ∼20 nm. Gene-encoding like fibers show slightly increased persistence length as compared to randomly distributed life-like fibers with NFRs, indicating that localizing short linkers near the 5′ NFR stabilizes the fiber slightly. Error bars depict standard deviations, which are computed across 1,000 conformations taken from the last 10 millions steps of several independent trajectories (See Methods for details).