Table 2:
Designation | Composition Linker Length (bp) | Linker Length Sequence (bp) | Ref | |
---|---|---|---|---|
1 | Uniform | 100% 18 (NRL = 165) | Uniform (18,18,18,18,18,18,18,18,18,18,...) | 41 |
2 | Uniform | 100 % 27 (NRL = 174) | Uniform (27,27,27,27,27,27,27,27,27,27,...) | 41 |
3 | Life-like | 70% 18, 20% 27, 10% 36 | 27 36 18 27 18 27 27 18 27 36 18 18 18 18 36 18 18 18 36 27 18 18 18 36 36 36 27 18 18 18 36 18 18 18 27 27 36 18 27 27 27 36 18 27 18 18 36 18 27 18 27 36 18 18 36 36 27 27 27 36 18 27 18 27 18 18 27 18 27 36 18 27 18 18 18 27 18 36 27 18 18 27 27 27 27 18 27 36 36 27 18 18 27 36 18 18 27 18 27 27 |
23 |
4 |
Life-like with NFRs Short NFRs: [72 bp] Long NFRs: [162 bp] |
70% 18, 20% 27, 10% 36 |
[162] 36 18 27 18 27 27 18 [72] 36 18 18 18 18 36 18 18 [72] 36 27 18 18 18 36 [72] 36 27 18 18 [72] 36 18 18 18 27 27 36 [72] 27 27 27 36 18 27 [162] 18 36 18 27 18 27 36 18 18 36 36 27 [72] 27 [162] 18 27 18 27 18 18 27 18 27 36 18 27 [72] 18 18 27 18 36 27 18 18 27 27 27 27 18 27 36 36 27 18 18 27 36 18 [162] 27 18 27 27 |
15,23 |
5 |
Gene encoding-like Short NFRs: [72 bp] Long NFRs: [162 bp] |
70% 18, 20% 27, 10% 36 with short linkers at the 5′ end | 5′ [162] 18 18 18 18 18 27 36 [72] 18 18 18 27 18 36 27 27 [72] 18 18 18 36 18 27 [72] 18 18 18 27 [72] 18 18 18 18 18 27 36 [72] 18 18 36 27 18 27 [162] 18 18 18 27 18 36 27 18 27 18 27 36 [72] 18 [162] 18 18 18 27 18 18 18 18 27 36 18 27 [72] 18 18 18 18 18 27 18 18 27 18 36 27 18 18 36 36 18 18 18 27 36 18 [162] 18 18 18 18 3′ |
15,23 |
6 | Gene encoding-like | 70% 18, 20% 27, 10% 36 | ″ | 15,23 |
7 | Gene encoding-like +LH | 70% 18, 20% 27, 10% 36 | ″ | 15,23 |
8 | Random (7 pairs of linker lengths) | 50% x (18–71), 50% y (18–71) | Random (x,y,y,x,x,y,y,x,y,x,y...) | |
9 | Alternating (7 pairs of linker lengths) | 50% x (18–71), 50% y (18–71) | Alternating (x,y,x,y,x,y,x,y,x,y...) | 42 |