Table 1.
Primers and probes used for the molecular detection of the protozoan parasites under study. In bold the fluorophores and quenchers
| Parasite | Primer | Sequence (5'-3') | Target | Reference |
|---|---|---|---|---|
| Cryptosporidium spp. | CF | GTTTTCATTAATCAAGAACGAAAGTTAGG | 18S rRNA | [19] |
| CR | GAGTAAGGAACAACCTCCAATCTCTAG | |||
| CP | 6FAM/TCAGATACCGTCGTAGTCTTAACCATAAACTATGCC/TAMRA | |||
| SSUrRNAF | AGTGACAAGAAATAACAATACAGG | 18S rRNA | [34] | |
| SSUrRNAR | CCTGCTTTAAGCACTCTAATTTTC | |||
| GpF | GCCGTTCCACTCAGAGGAAC | gp60 | [34] | |
| GpR | CCACATTACAAATGAAGTGCCGC | |||
| Giardia duodenalis | GdF | CATGCATGCCCGCTCA | 18S rRNA | [17] |
| GdR | AGCGGTGTCCGGCTAGC | |||
| GdP | 6FAM/AGGACAACGGTTGCAC/MGB | |||
| GDHeF | TCAACGTYAAYCGYGGYTTCCGT | gdh | [33] | |
| GDHiF | CAGTACAACTCYGCTCTCGG | |||
| GDHiR | GTTRTCCTTGCACATCTCC | |||
| AL3543 | AAATIATGCCTGCTCGTCG | tpi | [32] | |
| AL3546 | CAACATTITCCGCAAACC | |||
| AL3544 | CCCCTTCATCGGIGGTAACTT | |||
| AL3545 | GTGGCCACCACICCCGTGCC | |||
| Entamoeba histolytica | EhF | GTTTGTATTAGTACAAAATGGCCAATTC | 18S rRNA | [17] |
| EhR | TCGTGGCATCCTAACTCACTTAGA | |||
| EhP | 6FAM/CAATGAATTGAGAAATGACA/MGB | |||
| Toxoplasma gondii | TgF | TCCCCTCTGCTGGCGAAAAGT | B1 gene | [25] |
| TgR | AGCGTTCGTGGTCAACTATCGATTG | |||
| TgP | 6FAM/TCTGTGCAACTTTGGTGTATTCGCAG/TAMRA | |||
| Cyclospora cayetanensis | CcF | TAGTAACCGAACGGATCGCATT | 18S rRNA | [31] |
| CcR | AAT GCC ACG GTA GGC CAA TA | |||
| CcP | HEX/CCGGCGATAGATCATTCAAGTTTCTGACC/DABCYL | |||
| Internal control (Aquaglyceroporine) | CiF | ACCGTCATGGAACAGCACGTA | AQGP | [30] |
| CiR | CTCCCGCAACAAACCCTATAAA | |||
| CiP | VIC/AGCATCTGTTCTTGAAGGT/NFQ-MGB |