Table 5.
Cellulase activities of C. buqueti in different tissues
| Tissues | Female | Male | Larvae | Substrates | |
|---|---|---|---|---|---|
| Endoglucanase | Mouthpart | 167.574 ± 56.143cA | 36.944 ± 7.116bB | 22.227 ± 2.293aB | Carboxy methyl cellulose (CMC) |
| Foregut | 584.753 ± 91.215aA | 168.065 ± 27.839aB | 0aB | ||
| Midgut | 547.303 ± 55.846aA | 198.834 ± 35.686aB | 228.197 ± 49.354aB | ||
| Hindgut | 119.618 ± 46.375bcA | 74.403 ± 10.015aA | 0aB | ||
| Total intestine | 333.488 ± 56.600abcA | 150.491 ± 25.473aA | 0aA | ||
| Mouthpart + total intestine | 237.788 ± 49.687bcA | 122.701 ± 41.611aA | 17.936 ± 4.6191aB | ||
| Exoglucanase | Mouthpart | 0aA | 54.314 ± 12.635aA | 30.288 ± 6.118aA | Microcrystalline celluose (MC) |
| Foregut | 0aB | 15.939 ± 3.643bA | 44.996 ± 4.774aA | ||
| Midgut | 5.021 ± 1.112aA | 76.401 ± 17.143aA | 84.693 ± 12.384aA | ||
| Hindgut | 27.639 ± 9.401aA | 214.597 ± 54.711bA | 0aB | ||
| Total intestine | 0aA | 37.614 ± 4.551bA | 0aA | ||
| Mouthpart + total intestine | 6.768 ± 2.796aA | 93.996 ± 19.370bA | 2.821 ± 1.155aA | ||
| β-glucosidase | Mouthpart | 34.231 ± 10.168bB | 185.758 ± 53.732bA | 7.575 ± 2.613aB | Salicin |
| Foregut | 29.294 ± 11.822bB | 239.177 ± 100.275bA | 0.4454 ± 0.107aB | ||
| Midgut | 235.814 ± 59.925aB | 1744.827 ± 50.604aA | 6.487 ± 1.058aB | ||
| Hindgut | 22.959 ± 7.533bA | 0bA | 0.4217 ± 0.111aA | ||
| Total intestine | 53.002 ± 17.693bA | 382.107 ± 124.953bA | 2.429 ± 0.669aA | ||
| Mouthpart + total intestine | 36.031 ± 10.066bA | 326.273 ± 117.143bA | 5.788 ± 0.447aA | ||
| Laccase | Mouthpart | 0.049 ± 0.001bB | 0.168 ± 0.003aA | 0.053 ± 0.003bB | 2,2,-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) |
| Foregut | 0.004 ± 0.001bA | 0.192 ± 0.042aA | 0.029 ± 0.001bA | ||
| Midgut | 0.111 ± 0.011bB | 0.372 ± 0.061aB | 5.101 ± 0.532aA | ||
| Hindgut | 0.065 ± 0.001bA | 0.113 ± 0.014aA | 0.003 ± 0.001bA | ||
| Total intestine | 0.738 ± 0.231aA | 0.376 ± 0.072aA | 0.699 ± 0.099bA | ||
| Mouthpart + total intestine | 0.121 ± 0.038bB | 0.256 ± 0.008aA | 0.061 ± 0.004bB | ||
| Manganese peroxidase | Mouthpart | 0.327 ± 0.068aA | 0.917 ± 0.228aA | 0.326 ± 0.037bA | 2,6-Dimethyl phenol (2,6-DMP) |
| Foregut | 0.322 ± 0.025aA | 0.235 ± 0.006aA | 0.057 ± 0.003bA | ||
| Midgut | 0.124 ± 0.027aB | 0.475 ± 0.069aB | 2.162 ± 0.453aA | ||
| Hindgut | 0.558 ± 0.1129aB | 1.372 ± 0.191aA | 0.068 ± 0.002abA | ||
| Total intestine | 0.152 ± 0.010aA | 0.737±0.171aA | 0.893 ± 0.195bA | ||
| Mouthpart + total intestine | 0.082 ± 0.016aC | 0.324 ± 0.027aB | 0.772 ± 0.023abA | ||
| Lignin peroxidase | Mouthpart | 0.146 ± 0.022bA | 0.459 ± 0.130bA | 0.013 ± 0.003bcB | Veratryl alcohol (VA) |
| Foregut | 0.013 ± 0.001bA | 0cA | 0.002 ± 0.001bcA | ||
| Midgut | 0.437 ± 0.061bA | 0.471 ± 0.034bcA | 0.043 ± 0.012aB | ||
| Hindgut | 0.051 ± 0.010abB | 1.453 ± 0.289aA | 0.008 ± 0.002cB | ||
| Total intestine | 0bA | 0.065 ± 0.014bcA | 0.026 ± 0.008bA | ||
| Mouthpart + total intestine | 0bB | 0.067 ± 0.016bcA | 0.012 ± 0.003bcA |
A total of 165 female imagos, 165 male imagos and 165 larvae were sampled to determine enzyme activity. Five biological replicates were conducted for each treatment. Descriptive data are expressed as mean ± SEM. Different lowercase letters in the same column indicate significant differences in the different intestinal sections at the 0.05 level (n = 5); Different capital letters in the same line indicate significant differences at different developmental stage at the 0.05 level (n = 5)