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. Author manuscript; available in PMC: 2019 Apr 22.
Published in final edited form as: Nat Genet. 2018 Oct 22;50(11):1553–1564. doi: 10.1038/s41588-018-0244-3

Fig. 1. ChRO-seq and leChRO-seq measure primary transcription in isolated chromatin.

Fig. 1.

(a) Isolated chromatin is resuspended into solution, incubated with biotinylated rNTPs, purified by streptavidin beads, and sequenced from the 3’ end. leChRO-seq degrades existing RNA, extends nascent transcripts an average of 100 bp, and sequences RNAs from the 5’ end. (b and c) Comparison between matched ChRO-seq and PRO-seq in 41,478 RefSeq annotated gene bodies (b) or at the peak of paused Pol II (c). (d) Comparison between ChRO-seq (top three tracks), PRO-seq (center), and H3K27ac ChIP-seq, DNase-I-seq, and RNA-seq (bottom). dREG-HD shows the raw signal for dREG (gray) and dREG-HD signal (dark red). The shaded background shows the type of RNA produced at each position (e) The distribution of read lengths from ChRO-seq (blue) and leChRO-seq (pink) in a 30 year old primary GBM.