Figure 3. Baicalein eliminates liver TICs resistant to mTORC1 inhibition.

TICs and Huh7 cells were treated in spheroid formation condition for 7 days and the surviving cells or spheroids were re-plated in normal tissue culture condition for another 3–5 days. Representative pictures of A) TICs, C) Huh7 and E) PLC/PRF/5 cells after 7 days of the treatments are shown. Surviving re-plated cells stained with crystal violet are shown in A), C) and E) respectively. BC chemosensitizes B) TICs, D) Huh7 and F)PLC/PRF/5 cells toward CCl779 as assessed by crystal violet staining and expressed as percent of control. IC₅₀ values are shown in parentheses. For quantitative data, means and SEMs are shown, and two-way ANOVA analysis was performed with Bonferroni post-test to determine the statistical significance. **p<0.01.