Figure 8. Baicalein’s chemosensitization for CCI-779 is specific to cancer.

A) mTICs and freshly isolated mHep and were treated with different concentrations of CCI779 with or without 60 μM of BC for 5 days (Left). Huh7 cells or carefully thawed hHep were plated over night and treated similar to mHep (Right). MTT assay was performed to measure viability. B) Frozen pellets of mTICs, mHep, human HCC cells and hHep were lysed in GTP binding buffer with three freeze-thaw cycles and GTP bound SAR1B and total SAR1B were measured with SAR1B antibody. C) TICs and freshly isolated mHep were treated with indicated compounds for 2 hr. The cells were lysed and immunoblot analysis was performed with indicated antibodies.