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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Brain Res. 2018 Apr 27;1702:54–73. doi: 10.1016/j.brainres.2018.04.030

Figure 2. Human macrophages express enzymes necessary for the synthesis of norepinephrine and degradation of catecholamines.

Figure 2

Human macrophages were generated from peripheral blood mononuclear cells that were isolated from whole blood by ficoll density centrifugation, then matured into monocyte-derived macrophages (MDM) by adherence and maturation for 6 days in 10 ng/mL M-CSF. (A) Expression of mRNA for MAO-A and MAO-B, COMT and DBH is seen in mRNA derived from MDM from 4 donors. (B) Analysis of protein lysates from MDM derived from 3 or 4 additional donors shows expression of MAO (antibody did not distinguish A and B), COMT, and DBH protein.