Fig. 3. The migration, proliferation and adhesion capacities of U87 cells incubated with different media in vitro.

a Transwell assay of U87 cells cultured for 24 h in different media (n ≥ 3) (serum-free medium, CD90^low CM and CD90^high CM). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Serum-free medium was used as a control. b CCK8 assay of U87 cells (n ≥ 3) to evaluate proliferation in different media in vitro. U87 cells were incubated in 0%DMEM, CD90^high CM and CD90^low CM. *P < 0.05, **P < 0.01, ***P < 0.001. Serum-free medium was used as a control. c Adhesion assay to estimate the effect of 0%DMEM, CD90^high CM and CD90^low CM on U87 cell adhesion. (n ≥ 3) *P < 0.05, **P < 0.01, ***P < 0.001. Serum-free medium were used as a control