Fig. 5. Conditioned media from CD90^high and CD90^low gbMSCs have different functions in vivo.

a Representative mice from intracranial xenograft experiments in which U87 cells with CD90^high CM (left) or U87 cells with CD90^low CM (right) were injected into the right frontal lobes of nude mice. Obviously, the sizes of the CD90^high CM group tumours were greater than those of their CD90^low CM counterparts. *P < 0.05. b Both the CD90^high CM and CD90^low CM tumour sections were stained with HE (×200, scale bars = 50 μm). In the CD90^high CM and CD90^low CM tumour tissues, IHC was employed to detect CD31 and Ki-67 expression (×400, scale bars = 25 µm). (n ≥ 3) *P < 0.05, **P < 0.01. c Survival curves of glioma-bearing mice. The survival times of mice implanted with U87 cells cultured with CD90^high CM were not significantly shorter than those of mice implanted with U87 cells cultured in CD90^low CM. d Double staining for CD105 (green) and CD90 (red) revealed that CD105⁺CD90⁻ cells were located in the vessel walls, whereas CD105⁺CD90⁺ cells were located around the tumour parenchyma. (×400, scale bars = 25 µm). e Kaplan–Meier survival curves for patients with low and high CD90 expression. The survival of glioma patients with different CD90 expression levels in TCGA database was not significantly different