Table 1.

Sequences of primers used for real-time PCR.

	Gene access number	Forward	Reverse
Sirt-1	309757	5′-TGATTGGCACCGATCCTCG-3′	5′-TGGATGCTCTCATCAGGACAG-3′
PGC-1α	83516	5′-AAGTGGTGTAGCGACCAATCG-3′	5′-AATGAGGGCAATCCGTCTTCA-3′
ATP5α1	65262	5′-GAGACTGGGCGTGTGTTAAG-3′	5′-CTCCTCTGCTTGAACATTCCTC-3′
COX-5b	94194	5′-ACCCTAATCTAGTCCCGTCC-3′	5′-CAGCCAAAACCAGATGACAG-3′
TGF-β1	59086	5′-CCTGCAAGACCATCGACATG-3′	5′-TGTTGTACAAAGCGAGCACC-3′
Collagen 1	29393	5′-TCAAGATGGTGGCCGTTACT-3′	5′-CATCTTGAGGTCACGGCATG-3′
TNF-α	24835	5′-TCATCCGTTCTCTACCCAGC-3′	5′-TACTTCAGCGTCTCGTGTGT-3′
MCP-1	24770	5′-ACCAGCCAACTCTCACTGAA-3′	5′-GCCAGTGAATGAGTAGCAGC-3′
Nrf-1	312195	5′-GAGTGACCCAAACCGAACA-3′	5′-GGAGTTGAGTATGTCCGAGT-3′
GCLC	25283	5′-GATGATAGAACACGGGAGG-3′	5′-CATTGGTCGGAACTCTACTC-3′
Nox4	85431	5′-TGTCTGCATGGTGGTGGTAT-3′	5′-CTTCAACAAGCCACCCGAAA-3′
GAPDH	24383	5′-CTGGAGAAACCTGCCAAGTATGAT-3′	5′-TTCTTACTCCTTGGAGGCCATGTA-3′

Sirt-1: NAD-dependent deacetylase sirtuin-1; PGC-1α: peroxisome proliferator-activated receptor gamma coactivation factor 1α; ATP5α1: ATP synthase 5a; COX-5b: cytochrome oxidase 5b; TGF-β: transforming growth factor β; TNF-α: tumor necrosis factor α; MCP-1: monocyte chemoattractant protein 1; Nrf-1: nuclear respiratory factor 1; GCLC: glutamate cysteine ligase catalytic subunit; Nox4: NADPH oxidase 4; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.