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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Nature. 2018 Aug 29;561(7721):137–140. doi: 10.1038/s41586-018-0454-y

Figure 5 |. Seeded aggregation of full-length 3R, but not 4R, tau by the sarkosyl-insoluble fraction from Pick’s disease brain.

Figure 5 |

a, Coomassie-stained SDS-PAGE of the 0N3R and 0N4R recombinant tau preparations used for seeded aggregation. Two additional recombinant tau preparations were carried out with similar results. b, Thioflavin T fluorescence measurements of 0N3R (red) and 0N4R (blue) recombinant tau following incubation with (triangles) or without (circles) the sarkosyl-insoluble fraction from the frontotemporal cortex of patient 4. The results are from three independent experiments using separate recombinant protein preparations. The sarkosyl-insoluble fraction from Pick’s disease brain efficiently seeded the aggregation of 3R, but not 4R, tau