Extended Data Table 2 |. Primary anti-tau antibodies used in this study.
Details of the primary Anti-tau antibodies used in this study. Abbreviations are WB, Western blot; EM, Immunogold negative stain electron microscopy; IHC, immunohistochemistry; TEM, immunogold negative-stain electron microscopy; WB, western blot.
| Name | Epitope | Supplier | Cat. number | Species | Type | WB dilution | EM dilution | IHC dilution | Validation |
|---|---|---|---|---|---|---|---|---|---|
| BR133 | N-terminus | In house | - | Rabbit | Polyclonal | 1:4,000 | 1:50 | - | 30 |
| BR134 | C-terminus | In house | - | Rabbit | Polyclonal | 1:4,000 | 1:50 | - | 30 |
| BR136 | R1 | In house | - | Rabbit | Polyclonal | 1:4,000 | 1:50 | - | Extended Data Fig. 6 |
| Anti-4R | R2 | Cosmo Bio | CAC-TIP-4RT-P01 | Rabbit | Polyclonal | 1:2,000 | 1:50 | 1:100 | Manufacturer's datasheet and Extended Data Fig. 6 |
| BR135 | R3 | In house | - | Rabbit | Polyclonal | 1:4,000 | 1:50 | - | 30 and Extended Data Fig. 6 |
| Tau C4 | R4 | Masato Hasegawa | - | Rabbit | Polyclonal | 1:2,000 | 1:50 | - | 7 and Extended Data Fig. 6 |
| RD3 | Rl/3 | Millipore | 05-803 | Mouse | Monoclonal | 1:4,000 | - | 1:3,000 | Manufacturer's datasheet |
| 12E8 | pS262 and/or pS356 | Peter Seubert | - | Mouse | Monoclonal | 1:100,000 | 1:50 | 1:1,000 | 47,48 |
| AT8 | pS202 and pT205 | Thermo | MN1020 | Mouse | Monoclonal | 1:1,000 | 1:50 | 1:300 | Manufacturer's datasheet |
| MCI | Discontinuous epitope (residues 7-9 and 313-322) | Peter Davies | - | Mouse | Monoclonal | - | 1:10 | - | 24 |