Extended Data Table 3 |. Cryo-EM data collection, refinement and validation statistics.
| NPF (EMDB-0077) (PDB 6GX5) | WPF (EMDB-0078) | |
|---|---|---|
| Data collection and processing | ||
| Magnification | x105,000 | x105,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e-/Å2) | 55 | 55 |
| Defocus range (μ) | −1.7 to −2.8 | −1.7 to −2.8 |
| Pixel size (Å) | 1.15 | 3.45 |
| Symmetry imposed | None | C2 |
| Initial particle images (no.) | 83,475 | 8,024 |
| Final particle images (no.) | 16,097 | 3,003 |
| Map resolution (Å) | 3.2 | 8 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3.2 to 3.5 | 8 to 12 |
| Helical rise (Å) | 4.78 | 4.7 |
| Helical twist (Å) | −0.75 | −0.6 |
| Refinement | ||
| Initial model used (PDB code) | de novo | n/a |
| Model resolution (Å) | 3.2 | n/a |
| FSC threshold | 0.5 | |
| Model resolution range (Å) | 3.2 | n/a |
| Map sharpening B factor (A2) | −57 | −200 |
| Model composition | n/a | |
| Non-hydrogen atoms | 2133 | |
| Protein residues | 282 | |
| Ligands | 0 | |
| B factors (Å2) | n/a | |
| Protein | 75.8 average | |
| Ligand | n/a | |
| R.m.s. deviations | n/a | |
| Bond lengths (Å) | 0.0175 | |
| Bond angles (Å) | 1.1678 | |
| Validation | n/a | |
| MolProbity score | 2.15 | |
| Clashscore | 5.96 | |
| Poor rotamers (%) | 4.94 | |
| Ramachandran plot | n/a | |
| Favored (%) | 95.65 | |
| Allowed (%) | 100 | |
| Disallowed (%) | 0 |