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. Author manuscript; available in PMC: 2018 Dec 21.
Published in final edited form as: Mol Cell. 2017 Dec 7;68(6):1134–1146.e6. doi: 10.1016/j.molcel.2017.11.006

Figure 5. p53-RS increases c-Myc activity on ribosomal biogenesis.

Figure 5.

A) p53-RS dramatically increases rRNA expression. Vector or p53-RS plasmid was introduced into Hep 3B cells. RNA levels were analyzed using RT-PCR and Q-PCR.

B) p53-RS increases 5sRNA and tRNA expression. Vector or p53-RS plasmid was introduced into Hep 3B cells. RNA levels were analyzed using RT-PCR and Q-PCR.

C) Knockdown of p53-RS reduces rRNA expression. Sip53 or SiMyc was introduced into PLC/PRF/5 cells that were harvested for RNA analysis by RT-PCR and Q-PCR.

D) Knockdown of wt p53 increases rRNA expression. Sip53 or SiMyc were introduced into HepG2 cells that were harvested for analysis of RNA levels by RT-PCR and Q-PCR.

E) p53-RS binds to c-Myc’s target promoters. Vector or p53-RS plasmid was introduced into Hep3B cells that were harvested for ChIP assay using anti-p53 antibodies, and p53-RS-bound DNAs were analyzed by Q-PCR.

F) CDK4 inhibitor represses rRNA expression. Vector or p53RS were transfected into Hep 3B cells. Cells were treated with 500nM PD033291 for 24 h before harvesting for RNA analysis by RT-PCR and Q-PCR.

G) Knockdown of p53-RS represses the expression of mRNAs for ribosomal proteins. SiP53 was transfected into PLC/PRF/5 cells that were harvested for mRNA analysis by RT-PCR and Q-PCR.

H) p53-RS increases the expression of mRNAs for ribosomal proteins. Vector or the p53-RS plasmid was introduced into Hep 3B cells that were harvested for mRNA analysis by RT-PCR and Q-PCR.