| Antibodies |
| Mouse monoclonal anti-p53 (DO-1) |
Santa Cruz Biotechnology |
Cat# sc-126 AB_628082 |
| Mouse monoclonal anti-p53 (DO-1) |
Santa Cruz Biotechnology |
Cat# sc-126 X |
| Rabbit polyclonal anti-p53 (FL-393) |
Santa Cruz Biotechnology |
Cat# sc-6243 AB_653753 |
| Normal mouse IgG |
Santa Cruz Biotechnology |
Cat# sc-2025 AB_737182 |
| Rabbit monoclonal anti-GAPDH |
Cell Signaling Technology |
Cat# 5174 AB_10622025 |
| Rabbit monoclonal anti-Histone H3 |
Cell Signaling Technology |
Cat# 4499 AB_10544537 |
| Mouse monoclonal anti-cdc2 (CDK1) |
Cell Signaling Technology |
Cat# 9116P AB_2074795 |
| Rabbit monoclonal anti-CDK2 |
Cell Signaling Technology |
Cat# 2546P AB_2276129 |
| Rabbit monoclonal anti-CDK4 |
Cell Signaling Technology |
Cat# 12790P AB_2631166 |
| Rabbit monoclonal anti-CDK6 |
Cell Signaling Technology |
Cat# 13331P |
| Mouse monoclonal anti-CDK7 |
Cell Signaling Technology |
Cat# 2916P AB_10827986 |
| Rabbit monoclonal anti-CDK9 |
Cell Signaling Technology |
Cat# 2316P AB_2291505 |
| Mouse monoclonal anti-CDK4 |
Santa Cruz Biotechnology |
Cat# sc-56277 AB_1121419 |
| Rabbit polyclonal anti-Phosphorylation of S249 |
This paper |
N/A |
| Mouse monoclonal anti-Flag M2 |
Sigma-Aldrich |
Cat# F3165 AB_259529 |
| Mouse monoclonal anti-Cyclin D1 |
Santa Cruz Biotechnology |
Cat# sc-20044 AB_627346 |
| Mouse monoclonal anti-Cyclin D3 |
Cell Signaling Technology |
Cat# 2936P AB_10841292 |
| Rabbit polyclonal anti-Pin1 |
Cell Signaling Technology |
Cat# 3722S AB_10692654 |
| Rabbit polyclonal anti-Pin1 |
Santa Cruz Biotechnology |
Cat# sc-15340 AB_2237080 |
| Mouse monoclonal anti-Pin1 |
Santa Cruz Biotechnology |
Cat# sc-46660 AB_628132 |
| Rabbit monoclonal anti-PARP |
Cell Signaling Technology |
Cat# 9532S AB_10695538 |
| Rabbit monoclonal anti-c-Myc |
Abcam |
Cat# ab32072 AB_731658 |
| Mouse monoclonal anti-GFP |
Santa Cruz Biotechnology |
Cat# sc-9996 AB_627695 |
| Mouse monoclonal anti-P21WAF1
|
NeoMarkers |
Cat#MS-891-P1 |
| Mouse monoclonal anti-beta-Actin |
Sigma-Aldrich |
Cat# A1978 AB_476692 |
| Mouse monoclonal anti-beta-Actin |
Santa Cruz Biotechnology |
Cat# sc-47778 AB_626632 |
| Mouse monoclonal anti-alpha-tubulin |
Sigma-Aldrich |
Cat# 00020911 AB_10013740 |
| Peroxidase AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting after IP |
Jackson ImmunoResearch |
Cat# 115-035-174 AB_2338512 |
| Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific |
Jackson ImmunoResearch |
211-032-171 AB_2339149 |
| Mouse IgG Isotype Control |
Thermo Fisher |
Cat# 31903 AB_10959891 |
| Rabbit IgG Isotype Control |
Thermo Fisher |
Cat# 31235 AB_243593 |
|
|
|
| Bacterial and Virus Strains |
| DH5a competent cells |
NEW ENGLAND BioLabs |
Cat# C2987H |
| BL21(DE3) competent cells |
NEW ENGLAND BioLabs |
Cat# C2527H |
| Stbl3 competent cells |
Thermo Fisher |
Cat# A10469 |
|
|
|
|
|
|
| Biological Samples |
|
|
| Hepatocellular carcinoma (HCC) tissue samples |
The First Affiliated Hospital of Nanchang University, Jiangxi, China. |
N/A |
|
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| JNJ-7706621 |
Selleckchem |
Cat# S1249 |
| U0126-EtOH |
Selleckchem |
Cat# S1102 |
| PD-0332991 (Palbociclib) |
Selleckchem |
Cat# S1116 |
| Nocodazole |
Millipore |
Cat# 487929 |
| Okadaic acid (OA) |
Cell Signaling Technology |
Cat# 5934 |
| Alkaline Phosphatase, Calf Intestinal (CIP) |
NEW ENGLAND BioLabs |
Cat# M0290S |
| ATRA Pin1 inhibitor |
Kunping Lu |
N/A |
| Importazole |
Sigma-Aldrich |
Cat#SML0341 |
| MG132 |
Selleckchem |
Cat#S8410 |
| Cycloheximide (CHX) |
Sigma-Aldrich |
C7698 |
| P53S-249-phos-peptide |
This paper |
N/A |
| P53S-249-non-phos-peptide |
This paper |
N/A |
| γ32P-ATP |
Perkin-Elmer |
NEG002A250UC |
| Human His-p53-WT |
This paper |
N/A |
| Human His-p53-S249 |
This paper |
N/A |
| Human His-p53-SA(S249A250) |
This paper |
N/A |
| GST-Rb(379-928) |
This paper |
N/A |
| CDK4/Cyclin D1 Active |
SignalChem |
Cat# C31-10G |
| CDK4/Cyclin D3 Active |
SignalChem |
Cat# C31-18G |
| CDK2/Cyclin A1 Active |
SignalChem |
Cat# C29-10BG |
| CDK6/Cyclin D1 Active |
SignalChem |
Cat# C35-18H |
| iTAq Universal SYBR-green Supermix |
Bio-Rad |
Cat# 1725125 |
| Critical Commercial Assays |
| Cell Counting Kit-8 |
Dojindo Molecular Technologies |
Cat#CK04 |
| Deposited Data |
| Raw and processed data - ChIP-on-CHIP analysis In PLC/PRF/5 cells |
This paper |
GSE100968 |
|
|
|
| Experimental Models: Cell Lines |
| Human: HKE293 cells |
ATCC |
CRL-1573 |
| Human: HEK293 cells with Flag/HA-vector |
This paper |
N/A |
| Human: HEK293 cells with Flag/HA-p53-S249 |
This paper |
N/A |
| Human: PLC/PRF/5 cells |
Tong Wu |
Tulane University |
| Human: HepG2 cells |
Tong Wu |
Tulane University |
| Human: Huh7 cells |
Tong Wu |
Tulane University |
| Human: Hep 3B cells |
ATCC |
HB-8064 |
| Human: BT-549 cells |
ATCC |
HTB-122 |
| Human: H1299 cells |
ATCC |
CRL-5803 |
| Human: H1299 cells with GFP-p53-RS cells |
This paper |
N/A |
| Human: Hep 3B cells with plenti6/V5-vector cells |
This paper |
N/A |
| Human: Hep 3B cells with plenti6/V5-p53-RS cells |
This paper |
N/A |
| Human: WI38 cells |
ATCC |
CCL-75 |
| Oligonucleotides |
| SiRNA: SiCyclin D1-1 |
Sigma-Aldrich |
SASI_Hs01_002139 09 |
| SiRNA: SiCyclin D1-2 |
Sigma-Aldrich |
SASI_Hs01_002139 08 |
| SiRNA: SiCyclin D3-1 |
Sigma-Aldrich |
SASI_Hs01_000501 84 |
| SiRNA: SiCyclin D3-2 |
Sigma-Aldrich |
SASI_Hs01_000501 85 |
| SiRNA: SiCdk4(h) |
Santa Cruz Biotechnology |
Cat# sc-29261 |
| SiRNA: SiPin1(h) |
Santa Cruz Biotechnology |
Cat# sc-36230 |
| SiRNA: Sip53(h) |
Thermo Fisher |
106141 |
| SiRNA: Sip53(h) |
Sigma-Aldrich |
SASI_Hs01_000563 96 |
| SiRNA: SiMyc |
Santa Cruz Biotechnology |
Cat# sc-29226 |
| RT-PCR primers |
This paper |
See Table S1
|
| Recombinant DNA |
| Plasmids: pIRESneo3-Flag/HA-p53-S249 |
This paper |
N/A |
| Plasmids: pET-30a-His-p53-WT |
This paper |
N/A |
| Plasmids: pET-30a-His-p53-S249 |
This paper |
N/A |
| Plasmids: pCMV-neo-Bam-CDK4-HA |
Addgene |
Addgene Plasmid #1876 |
| Plasmids: pcDNA3.1-Flag-p53 |
This paper |
N/A |
| Plasmids: pcDNA3.1-Flag-p53-S249 |
This paper |
N/A |
| Plasmids: pcDNA3.1-Flag-p53-K280 |
(Girardini et al., 2011) |
N/A |
| Plasmids: pcDNA3.1-p53-K280-4M (33A,46A,81A 315A) |
(Girardini et al., 2011) |
N/A |
| Plasmids: pcDNA3.1-p53-K280-4M-RS (33A,46A,81A 315A & S249) |
This paper |
N/A |
|
|
N/A |
| Plasmids: pET-30a-His-p53-S249A250 |
This paper |
N/A |
| Plasmids: GST-Rb(379-928) |
Jiandong Chen |
Moffitt Cancer Center |
| Plasmids: pcDNA3.1-p53-4M (33A,46A,81A,315A) |
(Girardini et al., 2011) |
N/A |
| Plasmids: pcDNA3.1-p53-4M-RS (33A,46A,81A 315A & S249) |
This paper |
N/A |
| Plasmids: pcDNA3.1-p53-4M-D249 (33A,46A,81A 315A & D249) |
This paper |
N/A |
| Plasmids: pcDNA3.1-Flag-Pin1 |
(Lee et al., 2009) |
N/A |
| Plasmids: plenti6/V5-p53-WT |
Addgene |
Addgene Plasmid #22945 |
| Plasmids: plenti6/V5-p53-S249 |
Addgene |
Addgene Plasmid #22935 |
| Plasmids: plenti6/V5-p53-H273 |
Addgene |
Addgene Plasmid #22934 |
| Plasmids: pEGFP-N1-p53-RS(S249) |
This paper |
N/A |
| Plasmids:pcDNA-3.1-HA-c-Myc |
(Wang et al., 2015) |
N/A |
| Plasmids:pcDNA-3.1-4XFlag-FBW7a |
(Liu et al., 2010) |
N/A |
|
|
|
| Software and Algorithms |
| PrimerX |
Carlo Lapid |
http://www.bioinformatics.org/primerx/ |
| Bio-Rad Image Lab |
Bio-Rad |
N/A |
| Bio-Rad CFX Manager |
Bio-Rad |
N/A |
| ImageJ |
NIH Image |
N/A |
|
|
|
| Other |
|
|
|
