Skip to main content
. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Dev Comp Immunol. 2018 Sep 24;90:176–185. doi: 10.1016/j.dci.2018.09.017

Figure 1. Identification of the PAO1 secreted proteins and purification of elastase B.

Figure 1.

(A) The secreted proteins of PAO1 was separated by SDS-PAGE, visualized by Coomassie Brilliant Blue R-250, followed by in-gel trypsin digestion and mass spectrometry analysis. The identified proteins are indicated. Lane M, marker proteins; lane S, PAO1 secreted proteins. (B) Lane 1, fraction collected from 100 mM NaCl elution on the second run of Q-Fast Flow Sepharose column. Lane 2, purified elastase B from the HPLC gel filtration column. The protein bands were visualized by silver staining.