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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Dev Comp Immunol. 2018 Sep 24;90:176–185. doi: 10.1016/j.dci.2018.09.017

Figure 2. PAO1 elastase B reduces silkworm phenoloxidase (PO) activity and melanization.

Figure 2.

(A) The hemolymph PO activity was measured after injection of purified PAO1 elastase B into the larval silkworm, with phosphate-buffered saline (PBS) and bovine serum albumin (BSA) as controls. (B) Q-Fast Flow Sepharose beads were incubated with purified elastase B or BSA and then injected into the larval hemocoel. The beads were recovered from dissected larvae for melanization inspection under a microscope 4 h later. Each dot represents an individual larva. (C) PO activity of hemolymph from the larvae injected with purified PAO1 elastase B, activated by incubation with curdlan for 30 min. (D) The hemolymph samples were collected from larvae at 24 h post injection of PAO1 elastase B or BSA, activated by curdlan, and then separated on 8% SDS-PAGE followed by immunoblotting using silkworm PPO-1 and −2 antibodies.