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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Dev Comp Immunol. 2018 Sep 24;90:176–185. doi: 10.1016/j.dci.2018.09.017

Figure 3. PAO1 elastase B induces the production of silkworm gloverins.

Figure 3.

(A) The silkworm hemolymph samples from larvae at 24 h after injection of PAO1 elastase B, PBS and BSA were subjected to bacterial growth inhibition assays. PAO1 was used as the indicating bacterium on the plates. The data was from five individual larvae. (B) Boiled hemolymph samples from the silkworm larvae 24 h post injection were separated on a 16% Tricine-SDS-PAGE and the bands were digested with trypsin and analyzed by mass spectrometry. The band corresponded to silkworm gloverin is indicated by an arrow. (C) Gloverin-2, −3, and −4 relative mRNA levels in the fat body collected from larvae at 12 h post injection were determined by quantitative real-time PCR. The samples from PBS-injected larvae were used as control. All analyses were performed with five biological replicates.