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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Dev Comp Immunol. 2018 Sep 24;90:176–185. doi: 10.1016/j.dci.2018.09.017

Figure 4. PAO1 lasB expresses in the hemolymph of silkworm larvae after infection.

Figure 4.

(A) The growth curves of different PAO1 strains in Luria-Bertani broth. WT, PAO1 wild type; ΔlasB, lasB deletion mutant; ΔlasB-C, ΔlasB complemented; ΔlasB-pBBR1MCS-5, ΔlasB-C control. (B) PAO1 lasB expression after injection into the larval silkworm. The gene expression was analyzed by semi-quantitative PCR with GAPDH acting as an internal control. (C) Elastolytic activity in the hemolymph of PAO1 infected-silkworm larvae. The dash lines represent activity in the hemolymph after injection of purified elastase B.