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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Dev Comp Immunol. 2018 Sep 24;90:176–185. doi: 10.1016/j.dci.2018.09.017

Table 1.

Oligonucleotide primers used in this study

Primers sequence (5’-3’)
ΔlasB
P.a lasB up F CCTGGGATCCGGGCTGATCAGCA A GTTC
P.a lasB up R GGTTGTACACGAGTTTGGACACGTCGATC
P.a lasB down F GTCCAAACTCGTGTACAACCGTGCGTTC
P.a lasB down R CCTGAAGCTTGAAACGGGTGATGCGATG
ΔlasB-C
P.a lasB full F CCTGAAGCTTGCGTCGGCCGAGTACTTC
P.a lasB full R CCTGGGATCCGAGCTTACAACGCGCTCG
lasB expression
P.a lasB F CAACCAGAAGATCGGCAAGTA
P.a lasB R CGAATTGGCCAACAGGTAGA
P.a GAPDH F GCAGGTAGTGGCGATCAAT
P.a GAPDHR GGTTCTGGTCGTTGGTGTAG
Quantitative real-time-PCR
B.m gloverin2 F ACGGACCTTCTGATTACGC
B.m gloverin2 R CATTCTTGTTCGCCCAGT
B.m gloverin3 F GACACGAGAATGGGAGGAG
B.m gloverin3 R AAGACCCTGGTGCCGTAA
B.m gloverin4 F CTTGACAAGAACACCCGCCT
B.m gloverin4 R GTCTTGAAGGGATCTTCTGGAT
B.m IF4A F TCTGGCATCATACCTTCTACAA
B.m IF4A R TCTGTGTCATCTTTTCCCTGTT
P.a aprA F AGTTCCAAGCTGGTGTTCTC
P.a aprA R CCTTCTCGTTGAGGTTGATCTT
P.a lasA F CGCTGAATGACGACCTGTT
P.a lasA R CTTTCGGGTTGATGCTGTAGTA
P.a lepA F AGGACTGGTCGGATACAGTT
P.a lepA R TTTACGTTGAGGCCGATGAG
P.apiv F ATACCCTGACCGTCGAACT
P.a piv R GGAGTCGGCGAAATACGATAC
P.a GAPDH F(Q) GCTGGTGTCGGTGGATTT
P.a GAPDH R(Q) AGCATGCGGTTGGAGAAG

The restriction enzyme sites BamHI and HindIII are underlined