Table 1.
Primers | sequence (5’-3’) |
---|---|
ΔlasB | |
P.a lasB up F | CCTGGGATCCGGGCTGATCAGCA A GTTC |
P.a lasB up R | GGTTGTACACGAGTTTGGACACGTCGATC |
P.a lasB down F | GTCCAAACTCGTGTACAACCGTGCGTTC |
P.a lasB down R | CCTGAAGCTTGAAACGGGTGATGCGATG |
ΔlasB-C | |
P.a lasB full F | CCTGAAGCTTGCGTCGGCCGAGTACTTC |
P.a lasB full R | CCTGGGATCCGAGCTTACAACGCGCTCG |
lasB expression | |
P.a lasB F | CAACCAGAAGATCGGCAAGTA |
P.a lasB R | CGAATTGGCCAACAGGTAGA |
P.a GAPDH F | GCAGGTAGTGGCGATCAAT |
P.a GAPDHR | GGTTCTGGTCGTTGGTGTAG |
Quantitative real-time-PCR | |
B.m gloverin2 F | ACGGACCTTCTGATTACGC |
B.m gloverin2 R | CATTCTTGTTCGCCCAGT |
B.m gloverin3 F | GACACGAGAATGGGAGGAG |
B.m gloverin3 R | AAGACCCTGGTGCCGTAA |
B.m gloverin4 F | CTTGACAAGAACACCCGCCT |
B.m gloverin4 R | GTCTTGAAGGGATCTTCTGGAT |
B.m IF4A F | TCTGGCATCATACCTTCTACAA |
B.m IF4A R | TCTGTGTCATCTTTTCCCTGTT |
P.a aprA F | AGTTCCAAGCTGGTGTTCTC |
P.a aprA R | CCTTCTCGTTGAGGTTGATCTT |
P.a lasA F | CGCTGAATGACGACCTGTT |
P.a lasA R | CTTTCGGGTTGATGCTGTAGTA |
P.a lepA F | AGGACTGGTCGGATACAGTT |
P.a lepA R | TTTACGTTGAGGCCGATGAG |
P.apiv F | ATACCCTGACCGTCGAACT |
P.a piv R | GGAGTCGGCGAAATACGATAC |
P.a GAPDH F(Q) | GCTGGTGTCGGTGGATTT |
P.a GAPDH R(Q) | AGCATGCGGTTGGAGAAG |
The restriction enzyme sites BamHI and HindIII are underlined