Post-enediyne PKS tailoring steps for TNM A (1) biosynthesis in S. sp. CB03234 enabled the formulation of a unified biosynthetic pathway for all members of the family of anthraquinone-fused enediynes, DYN (4) in M. chersina, TNM (1), UCM (2) in S. uncialis, and YPM (3) in M. yangpuensis, featuring 26 as a key common intermediate. Groups transformed in each of the tailoring steps are highlighted in red. Intermediates in brackets are not isolated. Dashed arrows indicate shunt metabolites isolated.