Figure 4.

Effect of dietary zinc on cortico-striatal AMPAR mediated EPSCs in WT and Shank3^{ex13–16−/−} mice. (A) Schematic of electrode placement in dorsolateral striatum for all electrophysiology experiments. (B) Shank3^{ex13–16−/−} induces a decrease in AMPAR EPSC amplitude, and this decrease is not altered when animals are fed a high zinc diet (WT_{30 ppm} n = 11 cells from nine animals, $Shank{3}_{30\text{ ppm}}^{\text{ex1}3-16-/-}$ n = 8 cells from seven animals, WT_{150 ppm} n = 14 cells from nine animals, $Shank{3}_{150\text{ ppm}}^{\text{ex1}3-16-/-}$ n = 13 cells from eight animals). (C) Example AMPAR mediated EPSCs from each animal genotype and zinc diet. (D) Frequency histograms of AMPAR EPSC amplitudes from WT and Shank3^{ex13–16−/−} mice fed normal (left) or high zinc diet (right), illustrating the shift towards smaller AMPAR EPSC amplitudes in Shank3^{ex13–16−/−} mice on either zinc diet. All data represent mean ± SEM. *p < 0.05, student’s t-test.