Figure 6.

Dietary zinc supplementation prevents long term potentiation (LTP) in the cortico-striatal pathway in Shank3^{ex13–16−/−} mice. (A–C) WT_{30 ppm}, WT_{150 ppm}, and $Shank{3}_{30\text{ ppm}}^{\text{ex1}3-16-/-}$ mice all express LTP in response to tetanic stimulation (paired t-test, WT_{30 ppm} n = 11 cells from six animals, $Shank{3}_{30\text{ ppm}}^{\text{ex1}3-16-/-}$ n = 16 cells from six animals, WT_{150 ppm} n = 7 cells from four animals). (D) No significant change in AMPAR EPSC amplitude was observed 30 min after LTP induction in Shank3^{ex13–16−/−} mice with dietary zinc supplementation ($Shank{3}_{150\text{ ppm}}^{\text{ex1}3-16-/-}$: paired t-test, n =11 cells from five animals). (E) Bar graph of average percent change in AMPAR EPSC amplitude measured at 30 min post LTP-induction. All data represent mean ± SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001, NS, not significant.