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. 2018 Oct 22;9:2307. doi: 10.3389/fimmu.2018.02307

Figure 2.

Figure 2

RORγt inhibition of activated antigen-specific CD4+ T cells reduces the expression of neutrophil-recruiting chemokines by intestinal epithelial crypts. (A) Schematic figure of experimental plan. CFSE-labeled PBMCs were stimulated with FrvX (n = 8) or YidX (n = 7) during 12 days. Responding CFSE−CD4+ T cells were sorted and re-stimulated with their cognate antigen and were treated or not with BI119. After 7 days supernatants were obtained from these cultures, and added to intestinal human crypts (n = 5). After overnight culture RNA of the crypts was extracted. Messenger RNA expression of (B) CXCL1, (C) CCL20, (D) CXCL8, and (E) CXCL10 was assessed by real-time polymerase chain reaction. Mean ± SEM. ns > 0.05, *P ≤ 0.05, **P<0.005.