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. 2018 Oct 22;9:1479. doi: 10.3389/fpls.2018.01479

FIGURE 7.

FIGURE 7

Genes involved in lipid biosynthesis and assembly pathways are differentially regulated by JcWRI1. (A) Expression levels of lipid biosynthesis pathway genes in endosperm of J. cucas plants overexpressing JcWRI1 line #33-1. The Jatropha Ubiquitin transcript served as an internal control for all RNA samples. The value was presented as fold of the level in #33-1 against WT control (mean ± SD, n = 3) with statistical analysis by Student’s t-test ( indicates p < 0.05). (B) Diagram of gene structure of AW-box containing promoters. Dark bars in promoter regions indicate AW-box and gray bars indicate AW-like box. Bar: 100 bp. (C) Identification of direct target genes of JcWRI1 by Chromatin immunoprecipitation (ChIP) assay with WRI1 antibody. Developing J. curcas endosperm (six WAF) was harvested for ChIP assays. The fold enrichment was expressed relative to JcTubulin2 which was used as an internal control. Amplification levels obtained with the individual primer pair served as background signal of JcWRI1 binding. The experiment was repeated twice with similar results. Values are mean ± SD of three technical replicates with statistical analysis by Student’s t-test ( indicates p < 0.05).