Figure 6.
The Blastocystis alternative oxidase (AOX) is sensitive to salicylhydroxamic acid and thenoyltrifluoroacetone. (A) Routine respiration was determined in Blastocystis cells diluted to a density of 1 × 106 cells/ml before the addition of the AOX inhibitor salicylhydroxamic acid (SHAM) in two sequential 2.4 mM doses. Following SHAM addition the succinate dehydrogenase (Complex II) inhibitor thenoyltrifluoroacetone (TTFA) was added to a final concentration of 5.4 μM. Shown are an average of four independent experiments. Error bars represent standard deviation. (B) Routine respiration was determined in Blastocystis cells diluted to a density of 1 × 106 cells/ml before the addition of two doses of the Complex II inhibitor TTFA was added to a final concentration of 5.4 and 11 μM. Following this the AOX inhibitor SHAM was added in two doses to a final concentration of 2.4 and 4.8 mM. Shown are an average of two independent experiments. Error bars represent standard deviation.