Figure 4.

Dexamethasone increases Serpina3n expression in skeletal muscle and its secretion into circulation. Mice were treated with dexamethasone injection at 5 mg/kg/day for 4 days. Serpina3n mRNA levels in TA (A) and protein levels in gastrocnemius (B) muscles of CTR and DEX‐treated mice. (C) Serpina3n protein levels in serum of CTR and DEX‐treated mice. (D) Fold change of Serpina3n protein abundance in different tissues in response to dexamethasone treatment; dotted line corresponds to the CTR relative value. For western‐blotting, 20 μg of proteins were loaded in each lane for each sample. Results are means ± SE (n = 6/group). Statistical analysis was performed using unpaired t‐test (^* P < 0.05, ^** P < 0.01 vs. CTR). DEX, dexamethasone.