Figure 9.

IGF‐I inhibits glucocorticoids‐induced myotube atrophy and Serpina3n mRNA expression. (A) Phase‐contrast representative images of CTR, DEX, IGF‐I, and IGF‐I + DEX treated C₂C₁₂ cells. Myotube diameter (B) and Serpina3n mRNA levels (C) of C₂C₁₂ cells after 48 h of dexamethasone (10⁻⁶ M) and/or IGF‐I (50 ng/mL). Scale bar corresponds to 100 μM. Results are means ± SE (n = 3). Statistical analysis was performed using one‐way ANOVA and Tukey post‐test (^* P < 0.05, ^*** P < 0.001 vs. CTR, ^° P < 0.05, ^°°° P < 0.001 vs. DEX, ^● P < 0.05 vs. IGF‐I). DEX, dexamethasone; IGF‐I, insulin‐growth factor I.