Figure 11.

Muscle hypertrophy caused by myostatin inhibition is associated with decreased Serpina3n expression and secretion. (A) Serpina3n mRNA levels in tibialis anterior muscle of Mstn KO and WT mice (n = 6/group), mTr‐FS (Follistatin), and WT mice (n = 4/group) and sActRIIB‐treated and saline‐treated mice (n = 6/group). (B) Serpina3n protein abundance in gastrocnemius muscle of Mstn KO and WT mice. (C) Serpina3n protein abundance in circulation of Mstn KO and WT mice. For western‐blotting, 20 μg of proteins were loaded in each lane for each sample. Results are means ± SE. Statistical analysis was performed using unpaired t‐test (^* P < 0.05, ^** P < 0.01, ^*** P < 0.001 vs. CTR or WT). Mstn KO, myostatin knockout; WT, wild‐type.