Table 2.
Differentially secreted proteins between CTR and DEX‐treated C2C12 cells identified by nano‐LC‐MS/MS analysis
| Accession | Peptides | Score | ANOVA | Fold | Change | Protein | Gene GN |
|---|---|---|---|---|---|---|---|
| Q60590 | 2 (2) | 50.85 | 0.0025 | 16.9 | I | Alpha‐1‐acid glycoprotein 1 | Orm1 |
| G3X8T9 | 6 (6) | 295.96 | 0.0000 | 13.5 | I | Serine (Or cysteine) peptidase inhibitor, clade A, member 3N | Serpina3n |
| Q545R0 | 1 (1) | 23.1 | 0.0077 | 2.9 | I | Catenin (Cadherin associated protein), alpha 1 | Ctnna1 |
| Q61805 | 4 (4) | 209.28 | 0.0293 | 2.4 | I | Lipopolysaccharide‐binding protein | Lbp |
| P98064 | 2 (2) | 97.84 | 0.0006 | 2.1 | I | Mannan‐binding lectin serine protease 1 | Masp1 |
| D6RGQ0 | 7 (7) | 202.68 | 0.0018 | 1.8 | I | Complement factor H | Cfh |
| Q62009 | 28 (28) | 1369.95 | 0.0007 | 1.8 | I | Periostin | Postn |
| P47931 | 2 (2) | 107.81 | 0.0428 | 1.4 | I | Follistatin | Fst |
| Q9CR35 | 5 (5) | 152.27 | 0.0118 | 1.4 | I | Chymotrypsinogen B | Ctrb1 |
| F6SLR4 | 1 (1) | 20.1 | 0.0264 | 1.3 | I | Testican‐2 | Spock2 |
| B2RQQ8 | 6 (6) | 194.08 | 0.0244 | 1.3 | I | Collagen alpha‐2(IV) chain | Col4a2 |
| P62869 | 1 (1) | 45.96 | 0.0303 | 1.3 | I | Transcription elongation factor B polypeptide 2 | Tceb2 |
| P28301 | 6 (6) | 213.05 | 0.0257 | 1.3 | I | Lysyl oxidase | Lox |
| Q9JII6 | 1 (1) | 18.97 | 0.0302 | 1.2 | I | Alcohol dehydrogenase [NADP(+)] | Akr1a1 |
| P27773 | 11 (11) | 571.29 | 0.0176 | 1.1 | I | Protein disulfide‐isomerase A3 | Pdia3 |
| Q07797 | 1 (1) | 23.94 | 0.0354 | 5.2 | D | Galectin‐3‐binding protein | Lgals3bp |
| Q61554 | 4 (4) | 183.93 | 0.0481 | 1.9 | D | Fibrillin‐1 | Fbn1 |
| G5E8G2 | 1 (1) | 19.81 | 0.0290 | 1.8 | D | MCG124600 | Usp17ld |
| P07141 | 2 (2) | 140.75 | 0.0393 | 1.6 | D | Macrophage colony‐stimulating factor 1 | Csf1 |
| Q9WV91 | 1 (1) | 44.24 | 0.0408 | 1.5 | D | Prostaglandin F2 receptor negative regulator | Ptgfrn |
| Q3TNY9 | 11 (11) | 741.35 | 0.0088 | 1.5 | D | Biglycan | Bgn |
| P12032 | 3 (3) | 189.92 | 0.0489 | 1.5 | D | Metalloproteinase inhibitor 1 | Timp1 |
| B1B0C7 | 9 (9) | 349.69 | 0.0047 | 1.4 | D | Basement membrane‐specific heparan sulfate proteoglycan core protein | Hspg2 |
| P10518 | 3 (3) | 122.88 | 0.0341 | 1.4 | D | Delta‐aminolevulinic acid dehydratase | Alad |
| B1AYJ9 | 1 (1) | 70.83 | 0.0064 | 1.3 | D | Obg‐like ATPase 1 | Ola1 |
| Q3UQ28 | 15 (15) | 699.77 | 0.0147 | 1.3 | D | Peroxidasin homolog | Pxdn |
I or D = increase (I) or decrease (D) of protein secretion in CM of DEX‐treated C2C12 compared with CTR. DEX, dexamethasone.