Fig. 1: polymerase chain reaction (PCR) targets at rRNA-ITS region. Trichinella quantitative real-time (qPCR) corresponds to nucleotides 2285 (TCN-ITS1 2307F ) to 2370 (TCN-ITS1 2411R). Conventional PCR/DNA sequencing analysis corresponds to nucleotides 2375 (TCN-ITS1 2407F) to 3519 (TCN-28S 3511R). Internal PCR 1 corresponds to nucleotides 2375 (TCN-ITS1 2407F) to 3006 (TCN-ITS2 3020R) and Internal PCR 2 corresponds to nucleotides 3006 (TCN-ITS2 302F) to 3519 (TCN-28S 3511R). T. nativa accession no. KP307962 was used as a reference.