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. 2018 Oct 29;113(11):e180305. doi: 10.1590/0074-02760180305

TABLE II. Oligonucleotide sequences used for detection and identification of Trichinella nativa, T. spiralis, T. murrelli and Trichinella T6.

Name Primer sequencesa Type of oligo - Reaction nt Targetb Referencec
TCN-ITS1 2307F GAG TGT GAC CAA AAT GAG AAA CC Primer - TaqMan 2285-2307 (ITS1) KP307962
TCN-ITS1 2411R CAA ACC TAT TGA AAC CCA AGC AC Primer - TaqMan 2357-2370 (ITS1) KP307962
TnatR VIC-AAC ACA AAA AAT AAA C-MGBNFQ Probe - TaqMan 2527-2342 (ITS1) KP307962
TsprlR FAM-AGC ACA TTA CAC TGC ACT-MGBFNQ Probe - TaqMan 2338-2355 (ITS1) KC006422
TmurR FAM-AAC ACA CTG AGC ACT ACA-MGBNFQ Probe - TaqMan 2335-2352 (ITS1) KC006408
TT6R NED-CAA ACA CTA AAA TAA-MGBNFQ Probe - TaqMan 2322-2336 (ITS1) KP307967
TCN-ITS1 2407F GGT CAA CCG CCA CGT CCA ATC Primer - PCR, sequencing 2375-2395 (ITS1) KP307962
TCN-28S 3511R CTC GCC GCT ACT TGG AGA ATT CG Primer - PCR, sequencing 2497-3519 (ITS2) KP307962
TCN-ITS2 3020F TGT CGA CGT TGC AGT GTG TG Primer - PCR, sequencing 2957-2976 (ITS2) KP307962
TCN-ITS2 3020R CAC ACA CTG CAA CGT CGA CA Primer - PCR, sequencing 2957-2976 (ITS2) KP307962
18SrRNA-F TAT GCG ACT ACC ATG GTG ATA AC Primer - SYBR Green qPCR 349-371 (18S) NA
18SrRNA-R CTG CCT TCC TTG GAT GTG GTA Primer - SYBR Green qPCR 423-443 (18S) NA
ESVF GTT CCA TGT GAA CAG CAG T Primer - SYBR Green qPCR 1srDNA (20)
ESVR CGA AAA CAT ACG ACA ACT GC Primer - SYBR Green qPCR 1srDNA (20)

a: primers are in 5’-3 ‘orientation; b: nucleotide binding position; c: GenBank accession references.