TABLE II. Oligonucleotide sequences used for detection and identification of Trichinella nativa, T. spiralis, T. murrelli and Trichinella T6.
Name | Primer sequencesa | Type of oligo - Reaction | nt Targetb | Referencec |
TCN-ITS1 2307F | GAG TGT GAC CAA AAT GAG AAA CC | Primer - TaqMan | 2285-2307 (ITS1) | KP307962 |
TCN-ITS1 2411R | CAA ACC TAT TGA AAC CCA AGC AC | Primer - TaqMan | 2357-2370 (ITS1) | KP307962 |
TnatR | VIC-AAC ACA AAA AAT AAA C-MGBNFQ | Probe - TaqMan | 2527-2342 (ITS1) | KP307962 |
TsprlR | FAM-AGC ACA TTA CAC TGC ACT-MGBFNQ | Probe - TaqMan | 2338-2355 (ITS1) | KC006422 |
TmurR | FAM-AAC ACA CTG AGC ACT ACA-MGBNFQ | Probe - TaqMan | 2335-2352 (ITS1) | KC006408 |
TT6R | NED-CAA ACA CTA AAA TAA-MGBNFQ | Probe - TaqMan | 2322-2336 (ITS1) | KP307967 |
TCN-ITS1 2407F | GGT CAA CCG CCA CGT CCA ATC | Primer - PCR, sequencing | 2375-2395 (ITS1) | KP307962 |
TCN-28S 3511R | CTC GCC GCT ACT TGG AGA ATT CG | Primer - PCR, sequencing | 2497-3519 (ITS2) | KP307962 |
TCN-ITS2 3020F | TGT CGA CGT TGC AGT GTG TG | Primer - PCR, sequencing | 2957-2976 (ITS2) | KP307962 |
TCN-ITS2 3020R | CAC ACA CTG CAA CGT CGA CA | Primer - PCR, sequencing | 2957-2976 (ITS2) | KP307962 |
18SrRNA-F | TAT GCG ACT ACC ATG GTG ATA AC | Primer - SYBR Green qPCR | 349-371 (18S) | NA |
18SrRNA-R | CTG CCT TCC TTG GAT GTG GTA | Primer - SYBR Green qPCR | 423-443 (18S) | NA |
ESVF | GTT CCA TGT GAA CAG CAG T | Primer - SYBR Green qPCR | 1srDNA | (20) |
ESVR | CGA AAA CAT ACG ACA ACT GC | Primer - SYBR Green qPCR | 1srDNA | (20) |
a: primers are in 5’-3 ‘orientation; b: nucleotide binding position; c: GenBank accession references.