Fig. (2).

Analysis of curcumin protection on RPTEC/TERT1 against KBrO₃ induced oxidative stress and DNA adduct formation.

Fig. (2a, b) Cells were seeded in 24 well plates until fully confluent. After 10 days, cells were treated with 0.1% DMSO (control), curcumin, KBrO₃ and a combination of KBrO₃ + curcumin. Intracellular concentration of H₂O₂ was detected using the Amplex Red Hydrogen Peroxide Assay Kit. The data represents three independent experiments, *= p< 0.05.

Fig. (2c, d) DNA adduct formation was investigated by measuring the intracellular concentration of the DNA adduct 8-OHdG, using the Highly Sensitive 8-OHdG Check ELISA kit following the manufacturer kit instructions. The data represents three independent experiments. *= p< 0.05, **= p<0.01, and ***=p <0.001.

Fig. (2e) Catalase gene expression was examined in KBrO₃ (5.5mM) treated RPTEC/TERT1 cells after 24h treatment by RT-PCR analysis (* = P < 0.05).