FIG 1.

Sequence similarity of PfEMP1 CIDRα1 domains. (A) Maximum likelihood tree (key bootstrap [n = 50] values are indicated on branches) of 885 CIDRα1 sequences (30 kDa) (generated in Lau et al. [23]) with the 43 variants included in this study either as immunogen or in antibody. Reactivity measurements are marked by dots, and variant names are given in green boxes. Named arches mark CIDRα1 sequence subtypes as defined in Lau et al. (23). (B) Pairwise amino acid sequence identity (ID, identical sequence) heat map of the CIDRα1 recombinant domains (19 kDa) used in this study as immunogens (I) and for antibody detection in ELISA (E) or in Luminex (all proteins listed) assays.