FIG 2.

CIDRα1 reactivity and EPCR binding inhibition of IgG from animals immunized with a single recombinant CIDRα1 domain. The subtypes and specific variants of the CIDRα1 domains used as immunogens and analytes are stated in each panel (“Analyte: Figure 1” indicates that IgG reactivity was measured against the 43 recombinant CIDRα1 domains listed in Fig. 1B). (A) The IgG reactivity to the 43 CIDRα1 domains (as mean fluorescent intensity [MFI] measured by Luminex assay) is shown grouped by the domain used for immunization (Im) and by the subtype of the analytes: 1, CIDRα1.1; 4, CIDRα1.4; 5a or 5b, CIDRα1.5; 6a or 6b, CIDRα1.6; 7, CIDRα1.7; 8a or 8b, CIDRα1.8; cd36, CD36-binding CIDR. Box plots show median reactivity with 25th and 75th percentiles, upper and lower adjacent values, and outliers of plasma from four immunized animals to each of the tested domains. (B) The abilities of elicited IgGs to inhibit EPCR binding (ELISA) of the domain used for immunization (Im) and other select CIDRα1 domains are shown grouped by the CIDRα1 subtype of the analytes, as indicated on the x axes.