TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant details | Reference or source |
|---|---|---|
| C. difficile VPI 10463 | A known very-high-level toxin producer | 39 |
| C. difficile R20291 | Wild type; PCR ribotype 027 | 40 |
| C. difficile UK6 | An epidemic strain (provided by Dale Gerding and Abraham L. Sonenshein) isolated in the United Kingdom | 44 |
| CD2001 | Nontoxigenic C. difficile | Michel R. Popoff, Institut Pasteur |
| CCUG37780 | Michel Delmée (UCL, Belgium) to the Culture Collection of the University of Gothenburg (CCUG), Sweden | |
| CCUG37785 | ||
| CCUG37787 | ||
| Vector pHis1525 | Cloning vectors; the xylose operon is used as regulatory element | 41 |
| pRPF144 | Based on the pMTL960 backbone, subcloned into pBL139 | 42 |
| pBL139 | E. coli-C. difficile shuttle vector | 43 |