FIG 4.

The InlB/c-Met signaling pathway is functional in hepatocytes. (A) HepG2, Hep3B, PLC5, and Huh7 cell lysates were subjected to Western blot analysis using anti-c-Met and anti-actin (loading control) antibodies. (B) Cells were exposed, or not, to 1.25 nM InlB for 5 min, and cell lysates were subjected to Western blot analysis using anti-Akt and anti-phospho-Akt antibodies. A representative Western blot is presented (n = 3). (C) HepG2 cells were incubated with BSA- or BSA/InlB-coated beads for 30 min at 37°C (MOI of 5). Results are expressed as the average percentage of internalization ± SEM (n = 4; *, P < 0.01; **, P < 0.001). (D) After infection with WT or ΔinlB bacteria (MOI of 20) for 30 min, HepG2 cells were lysed and lysates were subjected to Western blot analysis using anti-Akt and anti-phospho-Akt antibodies. A representative Western blot is shown (n = 3). MW, molecular weight.